Scientific publications

Publications in peer-reviewed journals


Asturiano, J.F., Sørensen, S.R., Pérez, L., Lauesen, P., Tomkiewicz, J., 2016. First production of larvae using cryopreserved sperm: Effects of preservationtemperature and cryopreservation on European eel sperm fertilization capacity. Reproduction in Domestic Animals, 51, 485-491.

Butts, I.A.E., Sørensen, S.R., Politis, S.N., Tomkiewicz, J. 2016. First-feeding by European eel larvae: A step towards closing the life cycle in captivity. Aquaculture 464: 451-458.

Da Silva, Filipa F.G., Støttrup, J.G., Kjørsvik, E., Tveiten, H., Tomkiewicz, J. 2016. I Interactive effects of dietary composition and hormonal treatment on reproductive development of cultured female European eel, Anguilla anguilla. Animal Reproduction Science 171:17-26.

Jolly, C, Rousseau, K, Prézeau, L., Tomkiewicz, J., Dufour, S., Pasqualini, C. Functional characterization of eel dopamine D2 receptors and involvement in the direct inhibition of pituitary gonadotropins. Journal of Neuroendocrinology, Doi: 10.1111/jne.12411. (In press)

Lafont, A.G., Rousseau, K., Tomkiewicz, J, Dufour S. 2016. Three nuclear and two membrane estrogen receptors in basal teleosts, Anguilla sp.: identification, evolutionary history and differential expression regulation. General and Comparative Endocrinology, DOI: 10.1016/j.ygcen.2015.11.021 (In press.)

Mazzeo, I., Giorgini, E., Gioacchini, G., Maradonna, F., Vílchez, M.C., Balochez, S., Dufour, S., Pérez, L., Carnevali, O., Asturiano, J.F., 2016. A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla. Journal of Fish Biology, doi:10.1111/jfb.13103

Müller, A.V., McEvoy, F.J., Tomkiewicz, J., Politis, S.N., Amigo, R.J.M. 2016. Ultrasonographic predictors of response of European eels (Anguilla anguilla) to hormonal treatment for induction of ovarian development.American Journal of Veterinary Research 77: 478-489.

Peñaranda, D.S., Morini, M., Tveiten, H., Vilchez, M.C., Gallego, V., Dirks, R.P., van den Thillart, G.E.E.J.M., Pérez, L., Asturiano, J.F., 2016. Temperature modulates testis steriodogenesis in European eel. Comparative Biochemistry and Physiology, Part A, 197, 58-67.


Rozenfeld, C., Butts, I.A.E., Tomkiewicz, J., Zambonino-Infante, J. Mazurais, D. 2016. Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L. Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology 191, 59-65.


Støttrup, J.G., Tomkiewicz, J., Jacobsen C., Butts, I.A.E., Holst, L.K., Krüger-Johnsen, M., Graver, C., Lauesen, P., Fontagné-Dicharry, S., Corraze, G., Heinsbroek, L.T.N., Kaushik, S. 2016. Development of broodstock diet to improve developmental competence of embryos in European eel, Anguilla anguilla. Aquaculture Nutrition 22: 725-737.

Sørensen, S.R., Tomkiewicz, J., Munk, P., Butts, I. A. E., Nielsen, A., Lauesen, P., Graver, C., 2016. Ontogeny of early life stages of captive-bred European eel. Aquaculture 456, 50-61.

Sørensen, S.R., Butts, I. A. E., Munk, P., & Tomkiewicz, J. 2016. Effects of salinity and sea salt type on egg activation, fertilization, buoyancy and early embryology of European eel, Anguilla anguilla. Zygote, 1-18.


Baeza, R., Peñaranda, D.S., Vílchez, M.C., Tveiten, H., Pérez, L., Asturiano, J.F. 2015. Exploring correlations between sex steroids and fatty acids and their potential roles in the induced maturation of the male European eel. Aquaculture 435, 328-335.

Baeza, R., Mazzeo, I., Vílchez, M.C., Gallego, V., Peñaranda, D.S., Pérez, L., Asturiano, J.F., 2015. Relationship between sperm quality parameters and the fatty acid composition of the muscle, liver and testis of European eel. Comparative Biochemistry and Physiology, Part A, 181, 79-86.

Bouilliart, M., Tomkiewicz, J., Lauesen, P., Adriaens, D. (2015). Musculoskeletal anatomy and feeding performance of pre-feeding engyodontic larvae of the European eel (Anguilla anguilla).  Journal of Anatomy 227, 325-340.

Burgerhout, Erik, Yuki Minegishi, Sebastiaan A. Brittijn, Danielle L. de Wijze, Christiaan V. Henkel, Hans J. Jansen, Herman P. Spaink, Ron P. Dirks, and Guido EEJM van den Thillart. 2015. Changes in ovarian gene expression profiles and plasma hormone levels in maturing European eel (Anguilla anguilla); Biomarkers for broodstock selection. General and comparative endocrinology, doi:10.1016/j.ygcen.2015.08.006.

Butts, I.A.E., Baeza, R., Støttrup, J.G., Krüger-Johnsen, M., Jacobsen, C., Pérez, L., Asturiano, J.F., Tomkiewicz, J., 2015. Impact of dietary fatty acids on muscle composition, liver lipids, milt composition and sperm performance in male European eel. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. 183, 87-96.

Gallego, V., Vílchez, M.C., Peñaranda, D.S., Pérez, L., Herráez, M.P., Asturiano, J.F., Martínez-Pastor, F., 2014. The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regimen. Reproduction, Fertility and Development. 27, 529-543.

Morini, M, Pasquier, J, Dirks, R, van den Thillart, G., Tomkiewicz, J., Rousseau. K., Dufour, S., and Lafont, A-G. Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. Plos-One 10(5), doi:10.1371/journal.pone.0126008.

Støttrup J., Tomkiewicz J., Jacobsen C., Butts I.A.E.,, Holst L.K., Krüger-Johnsen M., Graver C.,  Lauesen P, Fontagné-Dicharry S., Heinsbroek L., Corraze G. & Kausik S, 2015. Development of a broodstock diet to improve developmental competence of embryos in European eel, Anguilla Anguilla. Aquacult. Nutr. 13p., doi: 10.1111/anu.12299.

Vílchez, M.C., Santangeli, S., Maradonna, F., Gioacchini, G., Verdenelli, C., Gallego, V., Peñaranda, D.S., Tveiten, H., Pérez, L., Carnevali, O., Asturiano, J.F. Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis. Theriogenology, doi:10.1016/j.theriogenology.2015.07.011.


Baeza, R., Mazzeo, I., Vílchez, M.C., Gallego, V., Peñaranda, D.S., Pérez, L., 2014. Asturiano, J.F. Effect of thermal regime on fatty acid dynamics in male European eels (Anguilla anguilla) during hormonally-induced spermatogenesis. Aquaculture, 430, 86-97.

Butts, I. A., Sørensen, S. R., Politis, S. N., Pitcher, T. E., & Tomkiewicz, J. (2014). Standardization of fertilization protocols for the European eel, Anguilla anguilla. Aquaculture, 426, 9-13.

Dirks, R.P., Burgerhout, E., Brittijn, S.A., de Wijze, D.L., Ozupek, H., Tuinhof-Koelma, N., Minegishi, Y., Jong-Raadsen, S.A., Spaink, H.P., van den Thillart, G.E. 2014. Identification of molecular markers in pectoral fin to predict artificial maturation of female European eels (Anguilla anguilla). Gen. Comp. Endocrinol. 204C, 267-276.

Gallego, V., Martínez-Pastor, F., Mazzeo, I., Peñaranda, D.S., Herráez, M.P., Asturiano, J.F., Pérez, L., 2014. Intracellular changes in Ca2 +, K+ and pH after sperm motility activation in the European eel (Anguilla anguilla): Preliminary results. Aquaculture 418–419, 155–158.

Mazzeo, I., Peñaranda. D.S., Gallego, V., Baloche, S., Nourizadeh-Lillabadi, R., Tveiten, H., Dufour, S., Asturiano, J.F., Weltzien, F.A., Pérez, L., 2014. Temperature modulates the progression of vitellogenesis in the European eel. Aquaculture, 434, 38-47

Peñaranda, D.S., Mazzeo, I., Gallego, V., Hildahl, J., Nourizadeh-Lillabadi, R., Pérez, L., Weltzien, F.A., Asturiano, J.F., 2014. The regulation of aromatase and androgen receptor expression during gonad development in male and female European eel. Reproduction in Domestic Animals, 49(3), 512-521.

Politis, S.N., Butts, I.A.E., Tomkiewicz, J. 2014 Light impacts embryonic and early larval development of the Europeaneel, Anguilla anguilla. Journal of Experimental Marine Biology and Ecology 461, 407–415.

Sørensen, S.R., Skov, P.V., Lauesen, P., Tomkiewicz, J., Bossier, P., Schryver, D.S., 2014. Microbial interference and potential control in culture of European eel (Anguilla anguilla) embryos and larvae. Aquaculture 426, 1-8.

Sørensen, S. R., Tomkiewicz, J., & Skovgaard, A. (2014). Ichthyodinium identified in the eggs of European eel (Anguilla anguilla) spawned in captivity. Aquaculture, 426, 197-203.

van der Meeren, T., Karlsen, Ø., Liebig, P. L., & Mangor-Jensen, A., 2014. Copepod production in a saltwater pond system: A reliable method for achievement of natural prey in start-feeding of marine fish larvae. Aquacultural Engineering 62, 17-27


Burgerhout, E., Tudorache, C., Brittijn, S.A., Palstra, A.P., Dirks, R.P., van den Thillart, G.E.E.J.M., 2013. Schooling reduces energy consumption in swimming male European eels, Anguilla anguilla L. J. Exp. Mar. Biol. Ecol. 448, 66–71. 

Burgerhout E., Brittijn, S.A., Tudorache, C., de Wijze, D.L., Dirks, R.P., van den Thillart, G.E. 2013. Male European eels are highly efficient long distance swimmers: effects of endurance swimming on maturation. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 166, 522-527. 

Gallego, V., Carneiro, P.C.F., Mazzeo, I., Vílchez, M.C., Peñaranda, D.S., Soler, C., Pérez, L., Asturiano, J.F., 2013. Standardization of European eel (Anguilla anguilla) sperm motility evaluation by CASA software. Theriogenology 79, 1034–1040.

Heinsbroek L., Støttrup J.,  Jacobsen C.,  Corraze G.,  Kraiem M., Holst L.K., Tomkiewicz J., Kaushik S., 2013. A review on broodstock nutrition of marine pelagic spawners: The curious case of the freshwater eels (Anguilla spp.). Aquaculture Nutrition 19, 1-24.

Peñaranda, D.S., Mazzeo, I., Hildahl, J., Gallego, V., Nourizadeh-Lillabadi, R., Pérez, L., Asturiano, J.F., Weltzien, F.-A., 2013. Molecular characterization of three GnRH receptor paralogs in the European eel, Anguilla anguilla: Tissue-distribution and changes in transcript abundance during artificially induced sexual development. Mol. Cell. Endocrinol. 369, 1–14.

Støttrup, J.G., Jacobsen, C., Tomkiewicz, J., Jarlbæk, H. Modification of essential fatty acid composition in broodstock of cultured European eel Anguilla anguilla L. Aquaculture Nutrition 19(2), pp 172–185.

Sørensen, S.R., Gallego, V., Pérez, L., Butts, I., Tomkiewicz, J., Asturiano, J., 2013. Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla. Reprod. Domest. Anim. 48, 936-944.


Gallego V., Mazzeo I., Vílchez M.C., Peñaranda D.S., Carneiro P.F.C., Pérez L.,  Asturiano J.F., 2012. Study of the effects of thermal regime and alternative hormonal treatments on the reproductive performance of European eel males (Anguilla anguilla) during induced sexual maturation. Aquaculture vol 354-355 pp 7-16.
Abstract available here 

Heinsbroek L., Støttrup J.,  Jacobsen C.,  Corraze G.,  Kraiem M., Holst L.K., Tomkiewicz J., Kaushik S. A review on broodstock nutrition of marine pelagic spawners: The curious case of the freshwater eels (Anguilla spp.). Aquaculture Nutrition, accepted.

Henkel CV, Burgerhout E, de Wijze DL, Dirks RD, Minegishi Y, Jansen HJ, Spaink HP, Dufour S, Weltzien FA, Tsukamoto K, van den Thillart GEEJM, 2012. Primitive Duplicate Hox Clusters in the European Eel’s Genome. PLoS ONE 7(2).

Henkel C.V., Dirks R.P., de Wijze D.L., Minegishi Y., Aoyama  J., Jansen H.J., Turner B., Knudsen B., Martin M., Kenneth Hvam K., Boetzer  M., Pirovano W., Weltzien F.A., Dufour S., Tsukamoto K., Spaink H.P., van den Thillart GEEJM. First draft genome of the Japanese eel, Anguilla japonica, constructed via a versatile de novo assembly pipeline. Gene 511, 195-201.

Mazzeo I., Peñaranda D.S., Gallego V., Hildahl J., Nourizadeh-Lillabadi R., Asturiano J.F., Pérez L., Weltzien F.-A., 2012. Variations in the gene expression of zona pellucida proteins, zpb and zpc, in female European eel (Anguilla anguilla) during induced sexual maturation. General and Comparative Endocrinology vol 178 pp 338-346.
Abstract available here  

Minegishi Y,  Henkel CV,  Dirks RP, van den Thillart GEEJM, 2012. Genomics in Eels — Towards Aquaculture and Biology. Marine Biotechnology; DOI 10.1007/s10126-012-9444-5.

Minegishi  Y, Dirks RP, de Wijze DL, Brittijn SA, Burgerhout SA, Spaink HP, van den Thillart GEEJM, 2012. Quantitative bioassays for measuring biologically functional gonadotropins based on eel gonadotropic receptors. General and Comparative Endocrinology. 178, 145–152

Pasquier J., Lafont  A.G., Jeng S.R., Morini M., Dirks R.P., van den Thillart GEEJM, Tomkiewicz J., Chang C.F., Rousseau K.,  Dufour S.. Multiple kisspeptin receptors in early osteichthyans provide new insights into the evolution of this receptor family. PLoS ONE 7(11).

Pasquier J., Lafont A-G,  Tostivint H., Vaudry H., Rousseau K., Dufour S., 2012. Comparative evolutionary histories of kisspeptins and kisspeptin receptors reveal both parallel and divergent features. Frontiers in Endocrinology. doi: 10.3389/fendo.2012 00173.


Pasquier J., Lafont A.G., Leprince J., Vaudry H., Rousseau K., Dufour S., 2011. First evidence for a direct inhibitory effect of kisspeptins on LH expression in the eel, Anguilla anguilla. Gen. Comp. Endocrinol. 173, 216-225.

Pérez, L., Peñaranda, D.S., Dufour, S., Baloche, S., Palstra, A.P., van den Thillart, G.E.E.J.M, Asturiano, J.F., 2011. Influence of temperature regime on endocrine parameters and vitellogenesis during experimental maturation of European eel (Anguilla anguilla) females. Gen. Comp. Endocrinol. 174, 51-59.
Abstract available here 

Tomkiewicz, J.,  Kofoed,  T.M.N. and Pedersen, J.S., 2011. Assessment of testes development during induced spermatogenesis in European eel Anguilla anguilla. Marine and Coastal Fisheries: Dynamics, Management and Ecosystem Science 3, 106-118. 
Abstract available here 

Burgerhout E,  Manabe  R, Brittijn SA, Aoyama J, Tsukamoto K, and van den Thillart GEEJM, 2011. Dramatic effect of pop-up satellite tags on eel swimming. Naturwissenschaften vol 98(7), 631–634.

Burgerhout E, Brittijn SA, Kurwie T, Decker P, Dirks RP, Palstra AP, Spaink HP & Van den Thillart GEEJM, 2011. Anguilla anguilla. BMC Developmental Biology 11:16. and Anguilla australisFirst artificial hybrid of the eel species
22 MAY 2024